---
title: A Rapid, High-Throughput Recombinant Antibody Expression System for Therapeutic Antibody Discovery and Validation
description: Hybridoma cell lines have historically offered an efficient, low-cost means to produce large numbers of monoclonal antibodies.
---

[Science ](https://www.antibody.com/science)

# [A Rapid, High-Throughput Recombinant Antibody Expression System for Therapeutic Antibody Discovery and Validation](https://www.antibody.com/science/a-rapid-high-throughput-recombinant-antibody-expression-system-for-therapeutic-antibody-discovery-and-validation)

 Written by [Antibody Solutions Research Team](https://www.antibody.com/science/author/antibody-research-team) | 12/1/18 5:55 PM

Hybridoma cell lines have historically offered an efficient, low-cost means to produce large numbers of monoclonal antibodies. However, there are reasons that warrant recombinantly expressing an antibody at a preclinical, R&D stage. These reasons include the need to express antibodies from primary B cells, a surface display library, convert chimeric antibodies to fully human antibodies, humanization of rodent antibodies, and isotype switching. We have developed a recombinant expression system for drug discovery with the ability to express a large number of recombinant antibodies in a timely, cost-efficient manner.

#### Summary

Antibody Solutions has developed a recombinant antibody expression system that allows for a large panel of antibodies to be expressed in a highthroughput and cost-efficient manner. The benefits of this system are:

- One vector per H + L chain
- Large Panels of antibodies (10’s - 100’s) possible
- Proper folding & human glycosylation
- 96-well culture screening - expand positive hits only
- Antibody available throughout the discovery process
- FACS sorting ensures monoclonality
- Only one transfection needed

#### Download this poster

We invite you to [download our poster ](https://www.antibody.com/science-poster-rapid-high-throughput-recobinant-antibody-expression) that details this research study, including our antibody discovery strategy as well as the Spike protein reagents, screening assay formats, and more that we utilized.

### Authors

Michael Trang, Leonel Santibanez-Vargas, Keerthi Sindhu, Catherine Vo, Edgar Rodriguez, John Kenney Antibody Solutions, Sunnyvale, CA, USA

 

[View full post](https://www.antibody.com/science/a-rapid-high-throughput-recombinant-antibody-expression-system-for-therapeutic-antibody-discovery-and-validation)

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