Possible Ags include:

  • Proteins
    • Peptides
    • Cells
  • Small Molecules
    • DNA
    • Cell Membranes
    • Lipoparticles
    • Polysaccharides
    • Others

General Considerations:

The form of the target antigen (e.g., native, non-native, complexed, etc.) impacts the type and form of the antigen used for immunization, the immunization regimen, and the Ab screening format. The Ag used to immunize may be different than the Ag used to analyze the resulting Abs. Ags of smaller size enable a more focused Ab response to a specific site, whereas larger Ags will identify Abs that bind the target in the context of the overall environment or milieu.

Importance of Ab binding to Ag Native Structure by Application

AB-binding

Impact of Ag Size on Ab Development

Ag-size