Guidelines for Storage and Stability of Abs

  • Store Abs at a concentration ~0.5 mg/mL. At lower concentrations, store in the presence of a carrier protein such as 1 mg/mL bovine serum albumin.
  • For short-term storage <3 months, place antibodies at 2-6◦C. Dispense aseptically or store in the presence of an anti-microbial preservative (e.g., ProClin)
  • For long-term storage (e.g., >3 months), place Abs at <-18◦C in a freezer that is NOT frost-free. Storage at lower temperatures (e.g., <-60◦C) is desirable, if available.
  • While most Abs are stable to multiple rounds of freeze-thaw, some will form aggregates or precipitate after freezing and thawing. This may occur even when stored at at 2-6◦C. Adjusting the pH of the storage buffer ±1 pH unit from the pI of the protein. Alternatively, adding 50% glycerol may alleviate the problem but do not store at <-20◦C.
  • Do not freeze phycoerythrin conjugates.
  • Aliquot Abs to avoid cross-contamination.
  • Most Abs are stable at ambient temperature for at least a few days.
  • The ideal storage conditions and expiration dating for an Ab can only be determined by stability studies. However, as a general guideline, Abs stored at -20◦C, at ~1 mg/ml in a neutral pH buffer are stable for several years or more.

Recommended Storage Temperatures and Expiration Dates for Critical Reagents^

Critical Reagents

O'Hara, et. al., 2012, AAPS Journal DOI:10.1208/s12248-012-9334-9