Bioconjugation in antibody discovery can be used for a wide range of applications. It requires an informed and steady hand, however, in order for it to work its magic. That’s where we come in.
There are different strategies and chemistries involved in producing stable, successful bioconjugates. Even the most common bioconjugations used in antibody discovery require expertise to generate a quality reagent.
Simply put, achieving an optimal ratio of conjugated molecules can be the difference between success and failure in the use of the reagent and whether or not the bioconjugate is fit-for-purpose.
Multiple Applications That Multiply Value
Selecting the optimal conditions for bioconjugation depends on both the intended application of the molecules of interest, as well as their structure. For example, immunization with a low peptide-to-carrier protein ratio can result in a poor antibody response. Similarly, screening with an over- or under-conjugated antigen or antibody can impact the sensitivity of the reagent in an assay for binding activity.
Successful bioconjugations can yield value in a broad spectrum of applications, from antibody discovery and purification to immunoassays and therapeutics. Antibody Solutions offers many types of bioconjugations, including the ones listed in Table 1.
Table 1: Common Bioconguations
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Fit-For-Success Processes
Bioconjugations vary widely in the choice of co-molecules, moiety compatibility, the chemistry used, their impact on functional activity, and in their application. You must also control for the event that a given molecule — chosen for the purpose of achieving your research goals — doesn’t lost its critical function after being joined to its co-molecule. That’s why it’s so important to have an experienced hand at your side to ensure bioconjugations are successful and fit-for-purpose.
Our team of experts can help keep your project moving at full speed by ensuring the bioconjugate is suitable for your research effort. We don’t have to farm out that specialty — we have it right here, and we rely on our broad knowledge of antibody discovery to help unlock the right combination that matches your application.
Through our custom conjugation services for both monoclonal and polyclonal campaigns, we can help optimize your research efficiency by:
Adhering to appropriate and time-tested protocols
Verifying conjugation reactions by determining conjugation ratios of the co-molecules
Addressing solubility concerns and using low-immunogenicity linkers
Developing analytical assays for conjugation ratios and material testing
Drive Real Value From Bioconjugation
True to our fit-for-purpose philosophy, we tailor our methodology to your target molecule. The end result of that focus is that we can offer your team:
Time and cost savings
We know from broad experience which specific reagents and chemistries to rely on when creating a bioconjugate of the highest sensitivity. You don’t need to spend time optimizing a protocol for a single project.
Confidence
Our comprehensive approach includes co-molecule evaluation, optimization efforts, and robust quality control (in part by bioconjugation verification, including SDS-Gels for peptide conjugates and the use of absorbance and other analytical assays for conjugation ratios).
Flexibility
Though we operate from established protocols, we also recognize the unique value that can come from developing and using novel co-molecules and chemistries for bioconjugation.
With all the skills and experience you need under our roof, Antibody Solutions can help you identify the best bioconjugates to use for immunizations or screening purposes, and then leverage those materials in your ongoing research initiatives. Your Antibody Solutions project manager will work one-on-one with you and your colleagues to identify your specific needs and match them to the optimal conjugates.
Your Project and Our Expertise: A Productive Combination
Magic can happen when the right things are paired. We see it happen on an almost daily basis around here. So the next time you’re looking for the right bioconjugation expert to help move your research forward.
