Methods and Assays
Custom-Tailored Method Development
Antibody Solutions offers custom-tailored method development for most bioanalytical assays used in pharmaceutical and diagnostics R&D. We focus on antibody-driven solutions that consider your specific needs, whether that’s for an innovation, a focus on cost-per-run, reliability or reproducibility, or a scalable process for high-throughput screening. And we’ve developed assays to measure processes such as enzyme activity, antigen capture, cell proliferation and activity, and competitive protein binding.
Assay Methods Tuned to Your End Goals
ELISA
Our clients have come to rely on our considerable expertise in ELISAs, using them frequently in various antibody discovery workflows. We can perform a range of ELISAs, from single plates to hundreds of them. We customize each assay to ensure we're creating the correct detectors or secondary reagents, and may perform an ELISA in multiple confirmations (for example, we might run “a” on the bottom and “b” as the detector in one run, and then reverse the roles in a second run).
Flow Cytometry
Since our founding, we have been investigating and pioneering the application of flow cytometry to rapidly screen antibodies - so much so, that it is considered a core capability and enabling technology for antibody discovery. We’ve overcome the challenges associated with large-scale antibody screening by reliably and efficiently generating monoclonal antibody cultures and utilizing the right combination of reagents to create the most efficient primary screen possible. In addition to the straightforward cell binding assays, our suite of flow cytometers enable custom bead based assays, multiplexed analysis, and high throughput automated screening.
Biolayer Inerferometry (BLI)
Label-free biomolecular interaction analysis technologies sparked a paradigm shift in the field of antibody discovery, allowing for the real time observation and measurement of protein-protein interactions. As an early adopter of the technology, Antibody Solutions is recognized as an expert in the field of biolayer interferometry (BLI), leveraging the technology for kinetics analysis, affinity determination, epitope binning, and even function assays such as receptor ligand blocking.
Putting Assays to Work, Smartly
Having experience with assay methods will only get you so far: more important is having an acute understanding of potential applications. Antibody Solutions can leverage key assay methods in any number of applications, including:
Reporter Based Assays
If you're looking for a specific function beyond mere binding from your antibody candidates, such as an agonist that stimulates different biological pathways or an antagonist that neutralizes a specific biological pathway, you may need a reporter based assay.
These types of assays need to be created and optimized to work, often in less than ideal conditions such as with antibody supernatants or within complex matrices.
We can develop and optimize reporter based assays so that they can be used as a primary screening tool or as a validation tool once a panel of antibody candidates has been selected.
PK/PD Assays
Once you’ve discovered the right reagents for you PK/PD assay, you have to create the assay that utilizes those antibodies. AS can help you determine the optimal conditions for your assay in an R&D setting.
Potency Assays
Potency assays, which measure a drug’s ability to elicit dose-dependent responses, are usually required by regulatory agencies before the release of a drug product. Antibody Solutions offers critical reagent development for the creation of potency assays. We use biolayer interferometry (BLI) technology to speedily deliver clear and reliable data. Our extensive experience with potency assay method development and qualification offers another way to approach your project.
Assays and Methods that Pass Every Test
Whether used in diagnostics, therapeutics, or research reagents, our assay and methods services deliver solutions that are innovative, inexpensive, reliable and amenable to high-throughput screening. In other words, they can pack exactly the punch our clients want.
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